Cov Khoom Kawg
Tiv tauj peb
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Chij Peptide, Flag peptide tag, Ib qho fusion tag hu ua DYKDDDDK, muaj li yim amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) thiab suav nrog qhov chaw enterokinase-cleavage, tau tsim tshwj xeeb rau kev siv hauv immunoaffinity chromatography. Cov cim npe no ua rau muaj kev cuam tshuam nyob rau hauv cov xwm txheej uas tsis yog denaturing. Ntau cov tshuaj tiv thaiv uas tsom rau cov peptide no tau tsim. Ib qho tshuaj tiv thaiv zoo li no, hu ua M1, khi cov peptide nyob rau hauv lub xub ntiag ntawm divalent hlau cations, nyiam dua Ca²⁺. Elution tuaj yeem ua tiav siv cov neeg ua haujlwm chelating lossis hloov pauv los ntawm kev sib tw elution nrog ntau tshaj ntawm DYKDDDDK peptide dawb.
Specifications
|
Sequence ib tsab ntawv code |
DYKDDDDK |
|
Sequence peb tsab ntawv code |
Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys |
|
CAS Nr |
98849-88-8 |
|
Molecular Formula |
C41H60N10O20 |
|
Molecular Luj |
1012.4 |
|
Kev hloov kho |
N/A |
|
Purity |
Peak Area los ntawm HPLC Ntau dua lossis sib npaug li 98% |
|
Daim ntawv |
Lyophilized |
|
Ntev Cia |
- 20 degree lossis qis dua |
| Rau kev tshawb fawb siv nkaus xwb. | |
Cov ntaub ntawv:
1. Einhauer A, Jungbauer A. Lub FLAG peptide, ntau yam fusion tag rau purification ntawm recombinant proteins. J Biochem Biophys Methods. 2001 Nov 30; 49(1–3): 455–65.
Cim npe nrov: chij peptide, chij peptide lwm tus neeg
Koj Tseem Yuav Zoo Li
