Peptide Conjugated KLH, BSA lossis OVA

Cov peptides luv luv feem ntau yog me me dhau los ua kom lub cev tiv thaiv kab mob txaus. Yog li ntawd, nws yog ib qho tsim nyog los sib txuas cov peptide mus rau cov khoom thauj loj dua los txhawb lub zog tiv thaiv kab mob thiab tsim kom muaj qib siab ntawm cov tshuaj tiv thaiv tshwj xeeb. Biorunstar muab cov khoom siv hauv qab no feem ntau siv rau cov peptide conjugates:

KLH (keyhole limpet hemocyanin, MW 4.5 × 10⁵ - 1.3 × 10⁷ Da) yog tooj liab- muaj protein ntau cais los ntawmMegathura crenulatathiab feem ntau yog thawj -kev xaiv cov khoom noj protein vim nws cov tshuaj tiv thaiv kab mob siab. Peptides conjugated rau KLH feem ntau muaj tsawg solubility hauv dej vim cov protein ntau thiab cov qauv; Txawm li cas los xij, qhov no tsis cuam tshuam rau nws txoj kev tiv thaiv kab mob, thiab cov kev daws teeb meem huab tseem tuaj yeem siv rau kev txhaj tshuaj.

BSA (bovine serum albumin, MW 67 × 10³ Da) yog cov khoom lag luam nrov rau cov tshuaj tiv thaiv tsis muaj zog vim nws cov lysine residues. Ua me me, BSA yog dej ntau dua -soluble dua KLH thiab feem ntau yog siv hauv immunoassays. Txawm li cas los xij, txij li BSA feem ntau siv los thaiv cov kev sib khi tsis sib xws, BSA conjugates yuav tsum tsis txhob siv rau kev txhaj tshuaj yog tias qhov kev ntsuam xyuas siv BSA, vim qhov no yuav ua rau muaj qhov tsis zoo vim yog cov tshuaj tiv thaiv BSA hauv antisera.

OVA (ovalbumin, MW 45 × 10³ Da) yog cov protein cais tawm ntawm cov qe qaib. Nws feem ntau yog siv los tswj cov khoom noj protein kom paub tseeb tias cov tshuaj tiv thaiv kab mob tshwj xeeb rau lub hom phiaj peptide es tsis yog rau cov neeg nqa khoom protein (xws li KLH / BSA).

Feem ntau, kev xaiv ntawm conjugation chemistry nyob ntawm qhov muab antigen peptide ib ntus.