Biotinylated Peptides

 

Biotinylated peptides yog cov cuab yeej tseem ceeb hauv kev tshawb fawb txog biomedical vim lawv muaj zog thiab tshwj xeeb kev sib cuam tshuam nrog streptavidin, ua kom muaj kev cuam tshuam zoo rau ntau yam streptavidin-coated nto xws li hlaws, daim nyias nyias, iav slides, los yog microtiter daim hlau. Cov cuab yeej no muaj txiaj ntsig tshwj xeeb rau kev tshuaj ntsuam xyuas xws li Peptide ELISA, qhov tseeb immobilization xyuas kom rov tsim dua thiab raug.

 

Biotin Labeling

Biotin tuaj yeem txuas rau peptide N-terminus lossis C-terminus (ntawm lysine lossis cysteine). Vim muaj kev sib cuam tshuam tsis zoo ntawm biotin thiab avidin / streptavidin, nws yog ib qho tseem ceeb kom muaj cov molecule me me no ua ib lub cim rau immobilize peptide, los yog rub-down peptide los ntawm kev sib tov. Nco ntsoov tias lub spacer, xws li mini-PEG lossis Ahx (aminohexanoic acid), nruab nrab ntawm biotin thiab N-terminus muab kev ua tau zoo dua hauv kev siv biotechnological feem ntau.

 

Tagged Peptides

Peptide tags tau siv dav hauv kev qhia cov protein thiab kev ua kom huv, thiab tseem siv los kuaj xyuas cov protein los ntawm Western blot, ELISA, Chip, immunocytochemistry, immunohistochemistry, thiab ntsuas fluorescence.

Tag Peptide	Ib ntus	Saib
Flag Tag	DYKDDDDK	Anti flag antibody
HA tag	YPYDVPDYA	Anti HA antibody
Myc Epitope Tag	EQKLISEEDL	Anti-Myc tshuaj tiv thaiv
6 Nws lub ntsej muag tag	HHHHHH	pab kom huv huv
V5 Peptide	GKPIPNPLLGLDST	tshuaj tiv thaiv

 

Cell -peptides nkag mus

Cell-kev nkag mus peptides tau tsim los ua ib qho hauv vitro thiab hauv vivo xa cov vectors los siv los ua kom yooj yim hauv zos rau cov cellular compartments (membranes, cytoplasm).

 

cov npe ntawm qee cov CPPs:

Peptide	Ib ntus
PIB-2	KLPVM
Poly-Arginine	RRRRRRRRR
HIV-Tat (47-57)	YGRKKRRQRRR
SynB1	RGGRLSYSRRRFSTSTGRA
PTD-5	RRQRRTSKLMKR
MAP	KLALKLALKLALALKLAJ
CAD-2	GLWRALWRLLRSLWRLLWKA-NH-CH2-CH2-SH
Peb-1	ac-KETWWETWWTEWSQPKKKRKV-cya
MPG	Ac-GALFLAFLAAALSLMGLWSQPKKKRKV-NH-CH2-CH2-SH