Peptide-Drug Conjugates (PDCs) Synthesis

Peptide-Drug Conjugates (PDCs) muab txoj hauv kev ua tau zoo rau kev xa khoom los ntawm kev sib txuas cov receptor- tshwj xeeb peptides nrog cov khoom siv ua haujlwm. Lub tswv yim no pab txhim kho lub hom phiaj kev ua tau zoo thaum txo qis - cov txiaj ntsig ntawm lub hom phiaj, ua rau PDCs muaj txiaj ntsig ntau dua hauv kev tshawb fawb kho mob thiab tshuaj ntsuam xyuas.

Ntawm Biorunstar, peb txhawb nqa PDC cov haujlwm los ntawm kev tsim qauv thaum ntxov los ntawm kev sib xyaw thiab kev txheeb xyuas qhov tseeb, nrog rau kev tsom mus rau kev ntseeg siab, hloov pauv, thiab kev sib txuas lus meej thoob plaws hauv cov txheej txheem.

 

Peb Cov Txheej Txheem PDC

1. Targeting Peptide Design & Synthesis

Peb muab kev cai synthesis ntawm peptides nrog muaj zog affinity affinity rau tej receptors, feem ntau siv nyob rau hauv lub hom phiaj kev tshawb fawb.

Cov piv txwv zoo xws li cov kab ke xws li RGD, cRGD, TAT, thiab GnRH.

Muaj kev hloov kho:

Cyclization (disulfide lossis amide bond)

N- lossis C-terminal hloov kho

Kev koom ua ke ntawm D-amino acids thiab tsis yog -natural amino acids rau kev txhim kho kev ruaj ntseg

 

2. Payload Conjugation & Labeling

Peb txhawb kev sib txuas ntawm peptides nrog ntau yam kev tshawb fawb thiab tshuaj -txog payloads, nyob ntawm koj daim ntawv thov.

Cytotoxic / me me - cov khoom lag luam molecule:

MMAE / MAV

Camptothecin (CPT)

Osimertinib

Ciprofloxacin (CIP)

Fluorescent labeling (rau kev yees duab & taug qab):

FITC, FAM

Cy3 / Cy5

PAB 630/650

Kev them nyiaj yug Radiolabeling:

Chelators xws li DOTA thiab NOTA rau kev kho hluav taws xob lossis kev tshawb fawb duab

 

3. Linker Selection & Conjugation Strategies

Linker tsim plays lub luag haujlwm tseem ceeb hauv kev ntsuas kev ruaj ntseg thiab tswj kev tso tawm. Peb pab xaiv thiab siv cov kab txuas txuas tsim nyog raws li koj qhov project hom phiaj.

Cleavable linkers:

pH-sensitive

Enzyme-cleavable (piv txwv li Val-Cit)

Disulfide-raws li

Tsis yog -cleavable linkers:

PEG-raws li

Alkyl chains rau solubility thiab pharmacokinetic tuning

Txoj kev sib txuas:

Nyem chemistry (DBCO / Azide-Alkyne)

Thiol-maleimide (Cys{0}} tshwj xeeb)

NHS ester (N-terminus lossis Lysine)

 

Tshwj xeeb Case Study

Osimertinib-Conjugated Peptide Synthesis

Peb tau ua tiav ntau yam haujlwm uas cuam tshuam nrog kev sib txuas ntawmOsimertinib (EGFR inhibitor)txhawm rau tsom peptides, txhawb nqa lub hom phiaj xa khoom thiab oncology-kev tshawb fawb ntsig txog.

https://www.biorunstar.com/projects/osimertinib-conjugated-peptide.html

Camptothecin (CPT) & Ciprofloxacin (CIP) Labeled Peptide Synthesis

Peb tau tsim peptide conjugates raws li covtib lub hom phiaj ib ntus, incorporating txawv payloads xws liCamptothecin (CPT)thiabCiprofloxacin (CIP)rau ntau yam kev tshawb fawb siv, nrog rau oncology thiab antibacterial kev tshawb fawb.

https://www.biorunstar.com/projects/cip-cpt-peptide-synthesis-case.html

 

Quality Assurance & Delivery

Txhua qhov kev txiav txim PDC yuav raug tswj xyuas kom zoo kom ntseeg tau tias kev ncaj ncees ntawm kev sib txuas:

HPLC & MS:Kev pov thawj ntawm purity (txog 98%) thiab molecular hnyav.

Solubility Testing:Xyuas kom lub conjugate haum rau koj cov tshuaj lom neeg.

Kev ntsuas kev ruaj ntseg:Cov ntaub ntawv ruaj ntseg yooj yim muaj thaum thov.

 

Thov ib tsab ntawv rau koj qhov project PDC

Npaj kom txav koj qhov kev tshawb fawb mus tom ntej? Hu rau peb pab pawg kws tshaj lij rau kev sab laj dawb thiab cov lus pom tseeb.

Email:sales@biorunstar.com

Saib ntau rooj plaub: www.biorunstar.com/projects