Biotin Peptides

Dab tsi yog Biotinylated Peptides?

Biotinylated peptides yog peptides uas tau hloov kho kom suav nrog biotin moiety. Qhov kev hloov kho no siv qhov zoo ntawm qhov siab - kev sib raug zoo ntawm biotin thiab avidin lossis streptavidin, uas yog siv dav hauv kev siv tshuaj lom neeg thiab tshuaj lom neeg. Lub biotin molecule yog me me, ruaj khov, thiab tsis hloov pauv cov qauv lossis kev ua haujlwm ntawm peptide, thaum ua kom muaj txiaj ntsig zoo los yog nrhiav tau los ntawm avidin-raws li systems.

 

Kev hloov kho tshuaj
Biotin feem ntau yog qhia los ntawm NHS-biotin (N-hydroxysuccinimide biotin) reagent, uas cuam tshuam nrog thawj pawg amine pom nyob rau ntawm peptide's N-terminus lossis lysine residues, tsim kom muaj kev ruaj ntseg amide bond.

Biotin (Peptide)

COOH NH₂

| |

+---EDC/NHS--- → 活化羧基 (Activation) → 缩合 (Coupling)

↓ ↓

Biotin-CONH-Peptide

 

Kev sib koom ua ke
Cov biotinylated peptide tuaj yeem khi ruaj khov (Kd ~ 10⁻¹⁵ M) rau avidin lossis streptavidin, ua rau nws zoo tagnrho rau kev siv hauv kev ntsuam xyuas uas yuav tsum tau kuaj xyuas meej, ntes, lossis kev tsis sib haum xeeb.

Biotinylated Peptide Synthesis Cases

Peptide Sequence: Biotin-Ahx-YXXXXXRXRRR-NH2

Ntev: 11A

Purity: >95%

M.W: 1898.32

Peptide Sequence: Biotin-YXXXXXRXRRR--ERXXXDLXXXXXPVXXXXPRR

Ntev: 31A

Purity: >95%

M.W: 4145.89

 

Kev siv Biotinylated Peptides

Biotinylated peptides yog ntau yam cuab yeej hauv kev tshawb fawb thiab kev lag luam, nrog rau ntau yam kev siv, suav nrog:

Affinity Purification

Siv los ntxuav cov neeg koom tes xws li cov proteins, tshuaj tiv thaiv kab mob, los yog nucleic acids los ntawm immobilizing biotinylated peptide ntawm streptavidin hlaws.

ELISA thiab Western Blotting

Biotinylated peptides ua raws li kev sojntsuam txhawm rau txheeb xyuas cov kev cuam tshuam tshwj xeeb thaum ua ke nrog avidin-HRP lossis avidin- cov ntawv fluorescent.

Nto Plasmon Resonance (SPR)

Siv rau immobilizing peptides ntawm biosensor chips rau kev kawm sib cuam tshuam.

Tshuaj Discovery

Kev tshuaj xyuas cov neeg sib tw tshuaj los ntawm kev tshuaj xyuas lawv txoj kev khi rau biotinylated peptide lub hom phiaj.

Immunology

Biotinylated peptides yog siv los qhia cov epitopes, kawm antigen-antibody kev sib cuam tshuam, thiab ua T- kuaj cell.

Epitope Mapping rau Kev Txhim Kho Tshuaj Tiv Thaiv

Ib qho kev cai biotinylated peptide tau siv los txheeb xyuas cov kab mob antigenic epitopes ntawm cov kab mob kis kab mob, pab tsim cov tshuaj tiv thaiv.

Protein -Protein Interaction Studies

Biotinylated peptides immobilized ntawm streptavidin- cov phaj coated ua rau txoj kev tshawb fawb ntawm receptor-ligand kev sib cuam tshuam, qhia txog kev sib koom ua ke tseem ceeb rau kev txhim kho tshuaj.

Immunoassay Optimization

Hauv kev tshuaj ntsuam xyuas, biotinylated peptides tau ua haujlwm ua antigens los txhim kho qhov rhiab heev thiab tshwj xeeb ntawm ELISA rau kev kuaj mob biomarker.

 

Cov ntaub ntawv

1. Green, NM (1975).Avidin thiab streptavidin.Biochemical Journal, 131(3), 781-791.

2. Chaiet, L., & Wolf, FJ (1964).Cov khoom ntawm streptavidin, biotin -binding protein tsim los ntawm Streptomyces.Archives ntawm Biochemistry thiab Biophysics, 106(1), 1-5.

3. Finn, FM, & Titus, G. (1994).Biotinylation ntawm cov proteins hauv kev tshawb fawb thiab biotechnology.Anal Biochem, 218(1), 1-7.