Kev Cai Peptide Synthesis Services

 

Biorunstar yog lub tuam txhab ua haujlwm puv -peptide. Peb siv cov khoom siv-theem peptide synthesis, kev daws teeb meem-theem synthesis, thiab microwave synthesis chemistry los npaj koj cov khoom siv peptide. Peb ntxuav lub siab xav tau siv HPLC npaj, thiab cov qauv peptide zaum kawg yog rov ua dua tshiab nrog thim rov qab-theem HPLC los xyuas kom meej purity. Mass spectrometry yog siv los lees paub qhov peptide qhov loj, thiab HNMR cov ntaub ntawv txheeb xyuas tau muab los txheeb xyuas qhov tshwj xeeb me me sib xyaw-peptide complexes.

 

Txheej txheem cej luam ntawm peptide synthesis service range Scope

Ntev ntawm qhov sib lawv liag ntawm 2 mus rau 120 amino acids
Peptide ntau npaum li cas los ntawm mg -kg
Different levels of purity, crude, desalt, >70, >80, > 90, >95% and >98%
Cyclic Peptides - Cys rau Cys, Lub taub hau rau Tail, Internal Lactam
Yooj yim thiab complex peptide hloov kho
Ib hlis twg muaj peev xwm tshaj 1000 peptides
Aliquots ntawm qhov ntau thiab tsawg raws li tus neeg thov

 

Feem ntau cov kev cai peptides xaj los ntawm Biorunstar tuaj yeem xa tuaj tsis pub dhau 2-3 lub lis piam. Lub sij hawm txhuas feem ntau txawv nyob ntawm qhov ntev peptide, kom muaj nuj nqis, thiab nyuaj. Txhua peptide tau muab nrog cov kev ntsuas nruj nruj, uas suav nrog HPLC chromatograms los xyuas kom meej purity thiab loj spectral tsom xam kom paub meej tias tus kheej.

 

Peptide synthesis hloov ntawm C terminus

C-terminus	Qauv Qhia	C-terminus	Qauv Qhia
Amidation		CMK/FMKChloro(fuoro)-methylketones
Aldehyde		Ester (OME / OEt)
Cawv		Hydrazide
Cysteamide (Mercaptoacetamide)		OSU
AMC 7-amino-4-methylcoumarinyl		pNA (p{0}}Nitroanilide)
AFC 7-amino-4-(trifluoromethyl)-2-benzopyrone

 

Peptide synthesis hloov ntawm Nqhov kawg

N-terminus	Qauv qhia	N-terminus	Qauv qhia
Acetylation		3-Indolylacetic acid
Benzoyl (Bz)		MpaInAA (3-Mercaptopropyl)
CBZ (Benzyloxycarbonylation)		Succinylation
Bromoacetyl (Br{0}}Ac)		MCA6-Maleimidohexanoic acid
Kev tsim kho		Thioester
Hydroxamic acid

 

Peptide Amidation thiab Peptide Acetylation

Yog hais tias peptide originates los ntawm cov protein nyob rau hauv ib theem zuj zus, Nyob rau hauv cov protein, cov peptide fragment xaus yuav amides (CONH2), yog li amidating lub C-terminus thiab acetylating lub N-terminus mimics no ntuj lub xeev. Cov kev hloov kho no tshem tawm cov peptide tus nqi thiab pab tiv thaiv nws los ntawm enzymatic degradation.

 

Peptide Amidation.	Peptide Acetylation

 

Aldehyde peptide:peptide aldehydes muaj lub zog inhibition ntawm ntau yam enzymes. Tsis tas li ntawd, peptide aldehydes yog qhov txaus siab rau peptide nraub qaum hloov kho lossis ligation cov tshuaj tiv thaiv.

 

CMK / FMK sau npe peptide:Peptide chloromethylketones (CMK) yog cov muaj zog heev thiab irreversible inhibitors ntawm serine proteases. Muaj nyob ntawm peptide ib ntus.

 

Ester (UA/OEt) : Rau cov qauv-kev sib raug zoo (SAR), tshem tawm cov nqi, prodrug.

 

p-Nitro-Aniline: ib chromogen siv raws li colorimetric enzyme substrate nyob rau hauv ntau tus qauv enzyme kev soj ntsuam nyob rau hauv cuvettes.Excitation siab tshaj plaws yog 410 nm.

 

Formylated peptide:Cov tsev neeg formyl peptide receptor (Fpr) paub zoo rau nws txoj kev pab tiv thaiv kab mob tiv thaiv kab mob hauv tib neeg thiab nas leukocytes.

 

Cov roj (cholesterol). sib txuaspeptide:cov roj (cholesterol) - cov tshuaj sib xyaw ua ke yog cov tshuaj tiv thaiv kab mob thiab cov membrane fusion. Cov roj (cholesterol) tuaj yeem txuas mus rau peptide ntawm N- lossis C-terminal ntxig cysteine.

Cholesterol sib txuasntawm peptide C-terminal:

 

Fatty acid conjugated peptides:Fatty acid conjugated peptides tuaj yeem siv rau kev tiv thaiv kab mob los yog eukaryotic cell toxicity thiab lwm yam. fatty acids xws li Caprylic acid (C8), Capric acid (C10), Lauric acid (C12), Myristic acid (C14), Palmitic acid (C16) los yog Stearic acid (C18) thiab lwm yam conjugated ntawm peptide N-terminal los yog ntawm C-terminal ntawm lysine sab saw.

 

Linkers, Spacers siv nyob rau hauv peptide synthesis

Raws li kev tsim thiab daim ntawv thov, peb tuaj yeem ntxig ib qhov txuas / txuas ntawm cov cim npe, protein (KLH, BSA) Biotin, lossis fluorescent thiab peptides. Ib hom hydrophobic spacer yog aminohexanoic acid (Ahx), thiab ib qho hydrophilic spacer yog poly (ethylene) glycol (PEG).Lwm tus thov siv cov lus hauv qab no: xws li Gly, AEA, Ava, ANP Linker, Beta-Alanine, C12, GABA, thiab lwm yam ...

 

Lub npe	Length (Tus naj npawb ntawm Bonds)	Qauv
(2-Aminoethoxy) acetic acid (AEA)	6
4-Aminobutyric Acid (GABA)	5
5-Aminovaleric Acid (Ava)	6
Aminohexanoic acid (Ahx)	7
12 -amino-dodecanoic acid (c12)	13
3-Amino-3-(2-nitrophenyl) prepanoic acid (ANP Linker)
Beta-Alanine	4
Mini-PEG	9
PEG

 

 

Dab tsi ntsev daim ntawv yuav nyiam rau kev cai peptide?

 

Kev cai peptides synthesized los ntawm SPPS yog los ntawm lub neej ntawd xa raws li trifluoroacetic acid (TFA) ntsev. Qhov no yog vim tias TFA yog siv thaum lub sij hawm cleavage ntawm peptide los ntawm cov khoom txhawb nqa resin thiab deprotection ntawm amino acid sab chains, Tsis tas li ntawd, TFA feem ntau yog siv los ua ib feem ntawm cov khoom tsis rau HPLC purification ntawm peptides. Cov ntsiab lus TFA feem ntau yog li ntawm 10% mus rau 45%, nyob ntawm seb muaj pes tsawg tus Arg, Lys, thiab Nws cov seem nyob rau hauv ib ntus.

 

Hauv qee qhov kev ntsuam xyuas ntawm tes, kev tshawb fawb tsiaj, thiab kev tshawb fawb hauv tsev kho mob, TFA seem yuav ua rau cov kev sim ua tsis tiav. Cov kws tshawb fawb tuaj yeem xaiv tshem tawm TFA lossis hloov mus rau acetate lossis hydrochloride ntsev. Vim yog cov txheej txheem tshem tawm TFA ntxiv, tus nqi yuav nce li ntawm 20%. Biorunstar tuaj yeem muab TFA tshem tawm (nrog TFA<1% guarantee) and TFA convert it to acetate or HCl salts services ( with TFA<0.1% guarantee) .