Lub peptide yog tsim los ntawm ib ntu luv ntawm cov amino acids txuas nrog peptide bonds.
Peptide synthesis yog ib qho chaw ua haujlwm nyob rau hauv cov protein thiab peptide chemistry, uas feem ntau koom nrog qhov sib ntxiv ntawm cov amino acids nyob rau hauv ib qho kev txiav txim kom tsim cov peptide saw los ntawm kev siv tshuaj. Lub ntsiab synthesis txoj kev yog kua-theem thiab solid-phase synthesis. Muab piv rau LPPS, SPPS muaj qhov zoo ntawm kev ua kom nrawm dua, yooj yim dua, nrog tsawg dua kev cuam tshuam, thiab cov txiaj ntsig siab dua.
Tus qauv SPPS txoj kev, suav nrog Boc (benzyl) thiab Fmoc (tBu) cov tswv yim.
Boc (benzyl) lub tswv yim
Nyob rau hauv no synthesis mus kom ze, Chloromethylated, hydroxymethylated polystyrene thiab p-methoxybenzyl (PMA) resins yog siv los ua khoom txhawb nqa. Cov pab pawg -amino tiv thaiv nrog t-butoxycarbonyl (Boc), thaum sab saw pawg ntawm cov amino acids tiv thaiv nrog benzyl derivatives. Cov pab pawg Boc raug tshem tawm siv TFA los yog TFA zoo hauv CH2Cl2, thiab thaum kawg ntawm kev sib txuas, sab-chain tiv thaiv pawg thiab peptide-resin linkages raug tshem tawm nrog cov kua qaub zoo li anhydrous hydrofluoric acid (HF) lossis TFMSA.
Fmoc (tBu) Strategy
Nyob rau hauv no synthesis mus kom ze, Wang-resin, 2-Chlorotrityl Chloride Resin, Rink amide-AM Resin, Rink amide-MBHA resins yog siv los ua khoom txhawb nqa.Cov -amino pab pawg yog tiv thaiv nrog 9-fluorenylmethoxycarbonyl (Fmoc ), thaum sab saw pawg ntawm cov amino acids tiv thaiv nrog acid-labile tiv thaiv pawg. Cov pab pawg Fmoc raug tshem tawm siv 20% piperidine hauv DMF, thiab thaum kawg ntawm kev sib txuas, sab-chain tiv thaiv pawg thiab peptide-resin linkages raug tshem tawm nrog 1%-95% TFA. Fmoc SPPS yog tam sim no txoj kev nyiam rau peptide synthesis.