Ceg Peptide

 

Branched peptides yog ib hom peptide qauv uas muaj ntau yam peptide chains (nplooj) txuas los ntawm lub hauv paus tseem ceeb, feem ntau muab los ntawm lysine. Cov lysine core muab cov pab pawg ua haujlwm (- thiab ε-amino pawg) rau peptide saw txuas. Tus naj npawb ntawm cov ceg tuaj yeem sib txawv (piv txwv li, 2, 4, lossis 8), nyob ntawm seb muaj pes tsawg tus lysine tiers siv hauv kev sib txuas. Lawv qhov hnyav molecular siab, piv rau linear peptides, ua rau lawv haum rau kev txhaj tshuaj yam tsis tas yuav tsum muaj cov cab kuj protein sib txuas. Multiple Antigenic Peptide (MAP) system yog ib txoj kev siv dav uas siv cov lysine cores rau kev tsim tshuaj tiv thaiv thiab tshuaj tiv thaiv kab mob.

Yam ntxwv

1. High molar ratio ntawm peptide antigen rau core molecule
2. Txhais cov qauv tshuaj
3. Kev xaiv ntawm 4-sab ceg, 8 ceg, thiab lwm yam
4. Kev xaiv ntawm mono-epitope lossis di-epitope

Qhov zoo

1. Tsis tas yuav tsum tau siv cov khoom nqa khoom noj protein kom tshem tawm cov tshuaj tiv thaiv kab mob
2. Ua kom cov txheej txheem ntau ntxiv ntawm cov khoom nto
3. Enhanced nrhiav tau rhiab heev rau cov theem khoom immunoassay
4. Muaj nuj nqis rau kev tshawb fawb txog kev tiv thaiv kab mob thiab tshuaj tiv thaiv

Cov lus qhia

1. Qhov nruab nrab peptide ntev: 10-15 residues
2. Txaus siab rau hauv N-terminal lossis sab hauv peptide teb
3. Tsis haum rau hauv C-terminal peptide teb
4. Cysteines tuaj yeem ua rau qis qis
5. Tsis haum rau kev ua kom huv ntxiv ntxiv.