Isotope Labeled Peptide

 

Cov isotope-labeled peptides ruaj khov yog tsim los ua cov cuab yeej siv tau zoo rau kev ntsuas peptide ntau npaum li cas lossis cov qauv protein thiab kev txiav txim siab zoo siv huab hwm coj spectrometry (MS) lossis nuclear magnetic resonance (NMR) spectroscopy, vim lawv paub qhov sib txawv ntawm qhov hnyav molecular. Biorunstar tau cog lus rau qhov zoo tshaj plaws hauv kev tsim cov isotope-labeled peptides ruaj khov.

 

Label Options

2 H (deuterium)

15N

13C

15N thiab 13C ua ke

Cov npe hauv qab no yog cov feem ntau ruaj khov isotopically sau cov amino acids uas tuaj yeem siv rau kev sib txuas ntawm peptides nrog cov isotopes ruaj khov uas muaj carbon 13 (13C), Nitrogen 15 (15N) thiab Deuterium (2H). Lwm cov npe amino acids muaj nyob rau ntawm qhov kev thov.

 

Amino Acid	Code	Isotope	Qhov txawv loj	Isotopic enrichment
Alanine	Ala (A)	U- 13C3, U-15N	+4 Da	>99%
Arginine	Arg (R)	U- 13C6, U-15N4	+10 Da	>99%
Isoleucine	Ile (kuv)	U- 13C6, U-15N	+7Da	>99%
Leucine	Lus (L)	U- 13C6, U-15N	+7 Da	>99%
Lysine	Lys (K)	U- 13C6, U-15N2	+8 Da	>99%
Phenylalanine	Pheej (F)	U- 13C9, U-15N	+10 Da	>99%
Proline	Pro(P)	U- 13C5, U-15N	+6 Da	>99%
Valine	Val (V)	U- 13C5, U-15N	+6 Da	>99%

 

Yam ntxwv nta

Tsim ntawm koj peptide nrog qhov xav tau loj sib txawv los ntawm kev xaiv cov plural ruaj khov isotope-labeled amino acids

Ib qho yooj yim thiab muaj txiaj ntsig kom muaj nuj nqis ntawm ntuj peptide los ntawm MSA

Isotope tsis yog radioactive

Muaj peev xwm hloov kho ntau yam xws li disulfide daim ntawv cog lus

Kev soj ntsuam ncaj qha ntawm cov qauv ntawm lub cev muaj sia yam tsis muaj kev kho mob ua ntej