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Fluorophore thiab Quencher khub (FRET Peptides)

 

Fluorescence resonance zog hloov (FRET) yog ib qho cuab yeej piav qhia txog lub xeev lub zog hloov pauv los ntawm qhov pib zoo siab pub dawb mus rau tus neeg txais. Tus pub molecules feem ntau emit ntawm luv wavelengths uas sib tshooj nrog kev nqus ntawm cov neeg txais. Cov txheej txheem yog kev ncua deb - nyob ntawm kev sib cuam tshuam ntawm cov hluav taws xob zoo siab ntawm ob lub molecules yam tsis muaj emission ntawm photon. FRET peptides tau dav siv los ua cov substrates tsim nyog hauv kev tshawb fawb enzyme.

Biorunstar tuaj yeem npaj cov npe hauv qab no Fluorophore thiab Quencher khub:

Fluorophore

Quencher

Kev zoo siab

Emission

Saib

Abz
2-Aminobenzoyl

Dnp
2,4-Dinitrophenyl CAS TSIS TAU

320nm ua

420nm ua

product-292-140

Abz
2-Aminobenzoyl

NitroTyr
3 -Nitro-tyrosine

320nm ua

420nm ua

product-339-126

Mca
7-Methoxycoumarin-4-yl) acetyl

Dnp
2,4-Dinitrophenyl CAS TSIS TAU

325nm ua

392nm ua

product-383-173

Trp
Tryptophan

Dnp
2,4-Dinitrophenyl CAS TSIS TAU

280nm ua

360nm ua

product-367-146

EDANS
5-[(2-Aminoethyl) amino] naphthalene-1-sulfonic acid

Dabcyl 4 -([4'-dimethylamino)phenyl]azo)benzoyl

340nm ua

490nm ua

product-598-287

FAM
Carboxyfluorescein

Dabcyl 4 -([4'-dimethylamino)phenyl]azo)benzoyl

492nm ua

517nm ua

product-602-232