Kev cai Peptide Synthesis

Biorunstar tsom ntsoov rau kev muab siab -cov kev pabcuam peptide synthesis zoo rau cov kws tshawb fawb, cov tuam txhab tshuaj, thiab cov tsev kawm ntawv thoob ntiaj teb. Peb cov txheej txheem peptide kev txiav txim yog yooj yim thiab yooj yim los xyuas kom meej cov khoom xa tuaj.

 

Kev nug thiab kev sab laj

Xa peb koj cov peptide ib ntus, cov lus qhia tshwj xeeb, thiab lwm yam kev xav tau ntxiv (xws li purity, kom muaj nuj nqis, hloov kho, sau npe, thiab lwm yam). Peb pab neeg txhawb nqa kev pabcuam yuav ua haujlwm nrog koj los tshawb nrhiav txoj hauv kev los txhim kho cov tswv yim sib txuas rau koj qhov project.

 

📝 Quotation and Confirmation

Peb yuav muab cov ncauj lus kom ntxaws raws li koj xav tau. Thaum koj pom zoo, ntau lawm yuav pib tam sim ntawd.>>🔗Yuav tso qhov kev txiav txim li cas?

 

Kev Cai Peptide Synthesis Nqe

Ib tus nqi amino acid rau 6-40 mer peptides

	Crude	Desalted	>70%	>75%	>80%	>85%	>90%	>95%	>98%
1-4 mg	$4.50	$5.50	$6.00	$6.50	$8.00	$9.50	$10.00	$11.00	$15.00
5mg ib	$5.00	$6.00	$6.50	$7.00	$8.50	$10.00	$10.50	$11.05	$17.00
10mg ib	$5.50	$6.50	$7.50	$8.50	$11.00	$13.00	$14.00	$16.00	$20.00
20mg ib	$6.00	$7.00	$9.50	$12.50	$13.50	$15.00	$16.00	$18.00	$25.00
50mg ib	$8.00	$9.00	$12.00	$15.50	$21.00	$25.50	$27.00	$30.00	$40.00
100 mg	$10.00	$13.00	$18.00	$24.00	$30.00	$40.00	$45.00	$50.00	$60.00
500 mg	$20.00	$28.00	$24.00	$40.00	$48.00	$58.00	$65.00	$70.00	$80.00
1000 mg	$34.00	$42.00	$40.00	$64.00	$75.00	$80.00	$90.00	$110.00	$130.00

Cov nqi teev nyob rau hauv lub rooj yog kwv yees rau tus qauv linear peptides tsis muaj kev hloov kho, tshwj xeeb amino acids, los yog daim ntawv lo. Rau qhov luv nqi thiab cov lus hais meej, thov hu rau peb ntawmsales@biorunstar.com.

Kev txo nqi yog txiav txim siab los ntawm yam xws li peptide ntev, qhov nyuaj, thiab qhov loj ntawm qhov kev txiav txim.

 

Peb cov kev pabcuam Peptide suav nrog

➖ Standard linear peptides

Ntev ntawm qhov sib lawv liag ntawm 2 mus rau 120 amino acids
Peptide kom muaj nuj nqis los ntawm lab scale mus rau ndustrial -scale (mg -kg)

Ib hlis twg muaj peev xwm tshaj 1000 peptides
Aliquots ntawm qhov ntau thiab tsawg raws li tus neeg thov

🔵 Peptide Amidation thiab Peptide Acetylation

Yog tias peptide pib los ntawm ib qho protein nyob hauv ib theem zuj zus, cov peptide fragment qhov kawg ntawm cov protein yuav ib txwm yog amides (CONH₂). Yog li ntawd, amidating C-terminus thiab acetylating N-terminus mimics lub xeev ntuj. Cov kev hloov kho no tshem tawm cov peptide tus nqi thiab pab tiv thaiv nws ntawm enzymatic degradation.

Peptide Amidation.	Peptide Acetylation

🔴 Peptide synthesis hloov ntawm C terminus

C-terminus	Qauv Qhia	C-terminus	Qauv Qhia
Amidation		CMK/FMKChloro(fuoro)-methylketones
Aldehyde		Ester (OME / OEt)
Cawv		Hydrazide
Cysteamide (Mercaptoacetamide)		OSU
AMC 7-amino-4-methylcoumarinyl		pNA (p{0}}Nitroanilide)
AFC 7-amino-4-(trifluoromethyl)-2-benzopyrone

🔵 Peptide synthesis hloov ntawm N terminus

N-terminus	Qauv qhia	N-terminus	Qauv qhia
Acetylation		3-Indolylacetic acid
Benzoyl (Bz)		MpaInAA (3-Mercaptopropyl)
CBZ (Benzyloxycarbonylation)		Succinylation
Bromoacetyl (Br{0}}Ac)		MCA6-Maleimidohexanoic acid
Kev tsim kho		Thioester
Hydroxamic acid

🧪 Lwm Modified Peptide Synthesis

>>🔗Peptide Biotinylation	>>🔗Ceg peptides
>>🔗Peptide Phosphorylation	>>🔗Methylated peptides
>>🔗fluorescent dyes labeling	>> 🔗FRET Peptide
>>🔗Hydrocarbon-stapled peptides	>>🔗ruaj khov isotope-labeled peptides
>> 🔗D-amino acid peptides	>> 🔗Chelator peptides
>>🔗Conjugate carrier proteins	>>🔗Cys-Cys disulfide cyclization, taub hau-rau-tail cyclization

📚 Peptide Libraries Tsim thiab Synthesis

>>🔗Overlapping peptide tsev qiv ntawv	>> 🔗Scrambled peptide tsev qiv ntawv
>> 🔗Truncation peptide tsev qiv ntawv	>> 🔗Alanine Scanning Library
>> 🔗T- lub tsev qiv ntawv cell truncated	>> 🔗Positional Scanning Library

🔄 Peptide TFA desalted thiab hloov kev pabcuam

Biorunstar tuaj yeem muab TFA tshem tawm (nrog TFA<1% guarantee) and TFA convert it to acetate or HCl salts services ( with TFA<0.1% guarantee) .

 

Kev cai peptides synthesized los ntawm SPPS yog los ntawm lub neej ntawd xa raws li trifluoroacetic acid (TFA) ntsev. Qhov no yog vim tias TFA yog siv thaum lub sij hawm cleavage ntawm peptide los ntawm cov khoom txhawb nqa resin thiab deprotection ntawm amino acid sab chains, Tsis tas li ntawd, TFA feem ntau yog siv los ua ib feem ntawm cov khoom tsis rau HPLC purification ntawm peptides. Cov ntsiab lus TFA feem ntau yog li ntawm 10% mus rau 45%, nyob ntawm seb muaj pes tsawg tus Arg, Lys, thiab Nws cov seem nyob rau hauv ib ntus.

 

Hauv qee qhov kev ntsuam xyuas ntawm tes, kev tshawb fawb tsiaj, thiab kev tshawb fawb hauv tsev kho mob, TFA seem yuav ua rau cov kev sim ua tsis tiav. Cov kws tshawb fawb tuaj yeem xaiv tshem tawm TFA lossis hloov mus rau acetate lossis hydrochloride ntsev. Vim yog cov txheej txheem tshem tawm TFA ntxiv, tus nqi yuav nce li ntawm 20%. Biorunstar tuaj yeem muab TFA tshem tawm (nrog TFA<1% guarantee) and TFA convert it to acetate or HCl salts services ( with TFA<0.1% guarantee) .

 

Yuav Ua Li Cas Peptide Synthesis

Kev sib piv ntawm Peptide Synthesis Methods

Txoj kev	Qeb	Txoj Cai	Qhov zoo	Qhov tsis zoo	Daim ntawv thov
Khoom -Phase Peptide Synthesis (SPPS)	Tshuaj synthesis	Stepwise saw elongation ntawm cov khoom siv coupling -deprotection cycles (Fmoc/Boc).	Ua haujlwm siab; Haum rau luv peptides (tsawg dua lossis sib npaug rau 50 AA); Tau tshaj cov kev hloov kho	Tsawg efficiency rau ntev peptides; Risk ntawm truncation vim coupling tsis ua hauj lwm	Lab tshawb fawb, kev tsim tshuaj peptide
Ua kua-Phase Peptide Synthesis (LPPS)	Tshuaj synthesis	Peptide chain extension lossis ntu condensation hauv kev daws.	Siab nruab nrab purity; Scalable; Ua tau zoo rau 2-6mers luv peptide	Tedious cov kauj ruam; Tsawg tsim rau ntev peptides	Muaj luv peptide ntau lawm
Enzymatic Synthesis	Semi - tshuaj	Protease-catalyzed peptide daim ntawv cog lus tsim.	Tsis muaj pab pawg tiv thaiv; Siab stereoselectivity; Eco-tus phooj ywg	txwv los ntawm substrate tshwj xeeb; Tus nqi enzyme siab	Bioactive peptide, ntsuab synthesis
Nyem Chemistry-Based Synthesis	Hybrid tswv yim	Nyem cov tshuaj tiv thaiv (piv txwv li, CuAAC) txuas peptide ntu.	High selectivity; Haum rau kev hloov kho nyuaj	Yuav tsum tau unnatural amino acids; Complex optimization	Tshuaj conjugates, dual{0}}labeled probes
Recombinant Qhia	Biosynthesis	Gene-raws li kev qhia nyob rau hauv host cell.	Zoo tagnrho rau ntev / qauv peptides; Txhawb kev tshaj tawm -kev txhais lus mods; Scalable	qeeb; Kev tsim cov noob yuav tsum tau ua; Kev suav nrog lub cev ua tau	Cov tshuaj tiv thaiv, tshuaj tiv thaiv kab mob, muaj protein ntau

Txoj Kev Xaiv Phau Ntawv Qhia

Kev xav tau	Txoj kev pom zoo	Lus hais
luv peptides (<50 AA) with complex modifications	SPPS	Kev tswj qhov chaw meej, siab -kev tshuaj ntsuam xyuas
Muaj- nplai 2–6mer luv peptide ntau lawm	LPPS lossis SPPS	LPPS yog scalable; SPPS rau automation
Glycosylated los yog lawm folded proteins	Recombinant qhia (eukaryotic)	Tshaj tawm - kev hloov pauv kev txhais tau zoo
Long peptide (>50 AA) nrog rau kev ua haujlwm lom neeg	Recombinant qhia los yog nyem chemistry	Kev nthuav qhia yog tus nqi- zoo; nyem suits modular tsim

Ntawm Biorunstar, feem ntau ntawm cov peptides kev cai yog tsim los ntawm peb cov kws tshaj lij chemists siv lub xeev - ntawm - cov - cov khoom siv hluav taws xob - cov txheej txheem kev sib txuas ua ke kom ntseeg tau siab purity thiab sib xws.

Kawm paub yuav ua li cas peptides synthesized siv SPPS.>>🔗Dab tsi yog peptide synthesis?

 

Peptide Purification thiab Kev Tswj Xyuas Zoo

Vim li cas peptides yuav tsum tau ntxuav?

Peptide purity yog qhov tseem ceeb uas cuam tshuam ncaj qha rau kev ua haujlwm, kev nyab xeeb, thiab kev ntseeg siab ntawm peptide hauv feem ntau ntawm kev tshawb fawb. Txhawm rau kom tsis txhob cuam tshuam los ntawm cov khoom lag luam uas tsis xav tau, Ntawm Biorunstar, peb muab qhov tseem ceeb tshaj plaws rau kev xa cov siab -purity peptides kom ntseeg tau tias muaj txiaj ntsig zoo thiab txhim khu kev qha.

Feem ntau Tsis xav tau Byproducts hauv Peptide Synthesis

Hom khoom lag luam	Kev piav qhia
❌ Cov khoom siv tiv thaiv tsis tiav	Cov pab pawg tiv thaiv seem (piv txwv li, Fmoc, Boc) tsis tshem tawm tag nrho, cuam tshuam kev ua haujlwm peptide.
❌ Ncua tseg	Peptides ploj ib los yog ntau dua cov amino acids vim tsis ua hauj lwm sib txuas.
❌ Deletion Sequences	Tsis muaj cov amino acids sab hauv -ntau dua los kuaj pom tab sis tseem tsis muaj roj ntsha.
❌ Sab - Cov Khoom Muag Hloov Kho Cov Khoom	Cov tshuaj tiv thaiv sab nraud ntawm cov saw hlau reactive (xws li oxidation ntawm Met, deamidation ntawm Asn).
❌ Racemization khoom	Tsim D-amino acids hloov L- cov ntawv, uas tuaj yeem hloov pauv kev ua haujlwm lossis txo qhov tshwj xeeb.
❌ Isomers lossis Conformational Variants	Peptide folding tsis raug lossis rov kho cov kab ke, tshwj xeeb tshaj yog nyob rau hauv cyclic lossis disulfide - txuas peptides.
❌ Aggregates los yog Adducts	Peptide dimers, trimers, los yog tsis yog - cov adducts tshwj xeeb tsim thaum lub sij hawm sib txuas los yog cleavage.
❌ Reagent Residue	Cov kab sib txuas ntawm cov tshuaj reagents (piv txwv li, TFA, cov kuab tshuaj, cov khib nyiab) uas nyob tom qab cleavage.

Cov txheej txheem rau Peptide Purification

Cov peptide tsis xav tau byproducts ( impurities ) feem ntau raug tshem tawm siv ib lossis ntau txoj kev ua kom huv.

🔧 Siab -Performance Liquid Chromatography (HPLC)

🌊 Rov qab-Phase Chromatography HPLC (RP-HPLC)

⚡ Ultra-High Performance Liquid Chromatography (UHPLC)

⚛️ Ion Txauv Chromatography (IEC)

⏳ Gel-Kev lim (Size Exclusion Chromatography)

In Biorunstar's purification process, we begin with Gel-filtration for pre-treatment when necessary, which includes steps such as desalting, buffer exchange, and removal of aggregates. The most commonly used method for peptide purification is RP-HPLC (Reverse-Phase High-Performance Liquid Chromatography). This technique ensures the isolation of high-purity peptides (e.g., desalted, >75%, >85%, >95%, >98%) los ntawm kev sib cais cov hom phiaj peptides los ntawm qhov tsis xav tau impurities raws li lawv cov hydrophobicity.

>>🔗 Pom zoo kev cai peptide purity Qib thiab kev tshawb fawb siv.

 

Txhua peptide raug tswj hwm nruj los ntawm HPLC thiab Mass Spectrometry (MS) tsom xam. Daim Ntawv Pov Thawj Paub Txog Kev Ntsuam Xyuas (COA) thiab daim ntawv tshaj tawm zoo suav nrog txhua qhov kev txiav txim, tshwj tsis yog cov peptides crude.

 

Kawm txog peptide synthesis tej yaam num peb tau ua tiav thiab xa.>>🔗Peptide Synthesis Cases.

 

Yuav ntsuas qhov Solubility ntawm Koj Kev Cai Synthetic Peptide li cas

Lub solubility ntawm peptide yog txiav txim los ntawm nws cov physicochemical zog (hydrophobicity, nqi, molecular hnyav) thiab hnyav ib puag ncig (polarity, pH, ionic zog). Lub ntsiab lus ntawm kev sib cais yog nyob rau hauv kev cuam tshuam cov kev sib cuam tshuam hauv nruab nrab (xws li, hydrophobic stacking, hydrogen bonds, ntsev txuas) thiab txhim kho cov kuab tshuaj -peptide kev sib cuam tshuam.

 

Los ntawm kev suav cov peptide tus nqi them (Net Charge ntawm pH 7), qhov nruab nrab hydrophilicity (Average Hydrophilicity), thiab qhov piv ntawm hydrophilic residues (Ratio of Hydrophilic) siv cov cuab yeej xws liBiorunstar Peptide Tshuab xam zauv, qhov nyuaj solubility hauv cov tshuaj aqueous tuaj yeem soj ntsuam ua ntej:

⚖️Net Charge ntawm pH 7

|Net Charge| Ntau dua los yog sib npaug rau 1: Tus nqi tseem ceeb txhim kho electrostatic repulsion hauv cov tshuaj aqueous, solubility zoo.

Net Charge Ntau dua lossis sib npaug rau 1: cais raws li peptide yooj yim; siv me ntsis acidic buffers.

Net Charge Tsawg dua lossis sib npaug rau -1: cais raws li acidic peptide; siv me ntsis alkaline buffers.

|Net Charge| < 1: Hluav taws xob nruab nrab lossis tsis muaj zog, ua rau micellization lossis sib sau ua ke; xav tau cov kuab tshuaj organic (xws li MeOH, DMSO, DMF).

💧 Ib nrab Hydrophilicity

Nruab Nrab Hydrophilicity Ntau dua lossis sib npaug rau 0: Zuag qhia tag nrho hydrophilic nyiam; zoo solubility dej.

Qhov nruab nrab Hydrophilicity <0: Zuag qhia tag nrho hydrophobic; xav tau cov kuab tshuaj organic (xws li DMSO, DMF).

📊Qhov ratio ntawm hydrophilic residues

Ratio Ntau dua lossis sib npaug li 50%: Soluble hauv dej lossis PBS ncaj qha.

Ratio < 50% : Tej zaum yuav xav tau cov kuab tshuaj organic lossis buffers nrog pH siab / qis.

Rau peptides uas muaj oxidation -sensitive residues (xws li, Cys, Met, Trp), zam cov kuab tshuaj los yog dissolution tej yam kev mob uas txhawb oxidation. xws li tsis kho DMSO lossis atmospheric oxygen raug. Siv cov pa oxygenated DMF, acidified aqueous TFA / formic acid nyob rau hauv inert gas, thiab suav nrog txo cov neeg ua hauj lwm (xws li, DTT) thaum tsim nyog.

 

Kev xa khoom thiab kev txhawb nqa

Peptides tau ua tib zoo ntim rau hauv lyophilized hmoov vials thiab xa los ntawmFedExkev xa khoom sai. Peb kuj muab kev txhawb nqa tom qab- xa khoom los pab nrog cov lus nug lossis teeb meem.

Kawm txog peb txoj cai tom qab - muag thiab xa rov qab.>>🔗 Txoj cai rov qab.

 

Cim npe nrov: kev cai peptide synthesis, kev cai peptide synthesis lwm tus neeg