Fluorescent Peptide

 

Fluorescent thiab dye-labeled peptides tau tsim rau hauv vivo biomedical imaging, protein binding, localization kev tshawb fawb, thiab ntau dua. Fluorescent dyes tuaj yeem txuas ncaj qha mus rau N- qhov kawg ntawm peptide (piv txwv li, FITC, feem ntau nrog cov aminohexanoic acid (Ahx) los yog -Ala txuas txuas nruab nrab ntawm fluorophore (fluorescein) thiab peptide's N-terminus), mus rau Lys lossis Cys sab chains C-terminus, lossis ntawm ntau txoj haujlwm hauv peptide.

 

Biorunstar muab ntau cov tshuaj fluorescent thiab dye-labeled peptides, xws li FITC, FAM, TAMRA, NBD (7-nitrobenz-2-oxa-1,3-diazole), Amc, Atto, Alexa Fluor, Bodipy, Cy3, Cy5, thiab ntau dua.Txhua lub fluorescent lossis zas xim -labeled peptide yog xa tawm raws li cov hmoov lyophilized hauv cov ntawv ci- cov raj npog.

 

FITC

Fluorescein isothiocyanate (FITC), ib qho amine-reactive derivative ntawm fluorescein dye, tau siv dav los sau cov peptides thiab cov proteins.Nws tuaj yeem sau npe rau ntawm peptide's N-terminus lossis ntawm lysine sab saw.

Fluorescein los yog zas xim	Xim	Absorption siab tshaj plaws	Emission siab tshaj plaws
FITC	Ntsuab	495nm ua	521nm ua

 

FAM

FAM yog cov tshuaj fluorescent feem ntau siv hauv confocal laser -scanning microscopy thiab flow cytometry applications.

 

Rhodamine B

Rhodamine B sawv cev rau ib qho ntawm ntau ntau ntawm rhodamine dyes, siv nyob rau hauv kev tshuaj ntsuam fluorescence.

Fluorescein los yog zas xim	Xim	Absorption siab tshaj plaws	Emission siab tshaj plaws
Rhodamine B	Txiv kab ntxwv	550nm ua	580nm ua

 

TAMRA

TAMRA yog ib qho ntawm cov txiv kab ntxwv fluorophores nrov tshaj plaws siv los sau cov peptides thiab cov proteins.

Fluorescein los yog zas xim	Xim	Absorption siab tshaj plaws	Emission siab tshaj plaws
Rhodamine B	Txiv kab ntxwv	544nm ua	576nm ua

 

NBD (7-nitrobenz-2-oxa-1, 3-diazole)

NBD-labeled peptides tau tshaj tawm tias yog ib qho kev xaiv nrov hauv kev tshawb fawb txog kab mob qog noj ntshav thiab cell.

Fluorescein los yog zas xim	Xim	Absorption siab tshaj plaws	Emission siab tshaj plaws
NBD	txiv kab ntxwv	486nm ua	543nm ua

 

AMC

AMC (7-amino-4-methylcoumarinyl), UV-excitable dye, siv nyob rau hauv enzyme kev soj ntsuam siv cuvettes los yog ntws cytometry.

Fluorescein los yog zas xim	Xim	Absorption siab tshaj plaws	Emission siab tshaj plaws
AMC	xiav	353nm ua	422nm ua

 

Cy3 thiab Cy5

Cy3 thiab Cy5 yog cov dyes uas tsis tshua muaj siab extinction coefficients thiab fluorescence, ua rau lawv tshwj xeeb tshaj yog haum rau rhiab heev localization soj ntsuam ntawm peptides hauv hlwb. Txawm li cas los xij, lawv qhov tsis zoo tseem ceeb yog qhov tsis ruaj khov ntawm cov molecules nyob rau hauv peptide synthesis tej yam kev mob, uas ua rau muaj kev sib piv tsis tshua muaj txiaj ntsig.