-
Xov tooj
+86-0755 2308 4243
-
Chaw nyob
Room 309, Meihua Building, Taiwan Industrial Park, No.2132 Songbai Road, Bao'an District, Shenzhen, Suav teb
-
Tug
FAQ
Cov txheej txheem synthesis dab tsi Biorunstar siv rau peptide synthesis?
RE: Biorunstar tau tsim cov txheej txheem kev daws teeb meem sib txawv kom tau raws li cov neeg siv khoom xav tau. Feem ntau, cov theem khoom Fmoc chemistry yog siv.
Koj xa peptides li cas? QC cov ntaub ntawv dab tsi yuav muab?
RE: Tag nrho cov peptides yuav raug xa los ntawm DHL lossis FedEx nthuav qhia xws li hauv cov hmoov lyophilized nyob rau hauv ib tus neeg zoo-labeled vials, ntawm chav tsev kub. Nrog rau kev zam ntawm crude peptides, uas tsuas yog yuav raug kuaj los ntawm MALDI-MASS spectrometry, tag nrho cov hluavtaws purified peptides tuaj nrog peb tes num (CoA), MS cov ntaub ntawv thiab cov ntaub ntawv HPLC. cov ntaub ntawv yuav raug muab uas muaj cov yam ntxwv tseem ceeb, xws li peptide ib ntus, purity, kom muaj nuj nqis, hloov kho, cov ntaub ntawv loj spectrum thiab HPLC cov ntaub ntawv.
Dab tsi yog lub sijhawm tig-ncig rau peptide synthesis? Nws yuav siv sij hawm ntev npaum li cas rau nkoj rau kuv?
RE: Peb lub sij hawm tig-ib ncig yog 2-3 lub lis piam rau tus qauv peptide hauv qab 30 amino acids. Lub sijhawm tig-ncig txawv nyob ntawm qhov ntev peptide, kom muaj nuj nqis, solubility thiab nyuaj. Feem ntau 3-5 hnub mus cuag cov kws tshawb fawb hauv lwm lub tebchaws.
Ntev npaum li cas koj tuaj yeem ua ke?
RE: Biorunstar muaj kev paub dav dav hauv kev tsim cov peptides ntev, txog li 130aa. Tsis zoo li ntau tus neeg muag khoom peptide uas tsuas yog nyiam ua peptides qis dua 30 lossis 40aa, Biorunstar muaj kev paub zoo hauv kev ua peptides xws li 40aa txog 90aa. Nws yog ib qho nyuaj rau synthesize peptides ntev, tshwj xeeb tshaj yog 100aa lossis ntev dua. Yog tias koj npaj yuav sib txuas ua ke ntawm 130aa lossis ntev dua, thov hu rau peb rau kev cai protein qhia thiab kev pabcuam purification.
Yuav ua li cas yog ib co teeb meem tshwm sim thaum lub sij hawm synthesis los yog purification txheej txheem?
RE: Txhua peptide muaj nws cov yam ntxwv tshwj xeeb. Yog tias qee qhov teeb meem tshwm sim thaum lub sijhawm sib xyaw ua ke dhau qhov peb xav tau, thiab peb tsis tuaj yeem xa koj cov peptide raws sijhawm, peb yuav qhia koj sai li sai tau. Los ntawm lub caij nyoog uas peb tsis tuaj yeem ua cov peptide, koj yuav tsis raug them nqi.
TFA ntsev daim ntawv, Acetate lossis HCl ntsev daim ntawv: Kuv yuav xaiv daim ntawv twg?
Los ntawm lub neej ntawd, peptides yog synthesized hauv TFA ntsev. Rau kev sim ntawm tes lossis cov ntaub so ntswg ntsig txog kev sim, koj yuav tsum xav txog kev muaj peptides tsim hauv acetate lossis HCl ntsev daim ntawv ntawm 98% lossis siab dua kom tsis txhob muaj cov lus teb txawv txav. Acetate lossis HCl ntsev tuaj yeem thov ntawm tus nqi ntxiv.
Lub txee lub neej ntawm peptide yog dab tsi?
RE: Peptides ruaj khov rau tsawg kawg ib xyoos yog tias khaws cia lyophilized hauv lub freezer. Yog tias peptides yaj, lub txee lub neej yuav raug txo. Yog tias peptides muaj ntau cov amino acids reactive uas tuaj yeem oxidized zoo li Trp, Met tab sis tshwj xeeb tshaj yog Cys, lub txee lub neej kuj yuav raug txo.
Cov peptide muaj ob peb cysteine thiab tuaj yeem yooj yim tsim cov nyiaj disulfide. Kuv yuav zam qhov no li cas?
RE: Yog hais tias cov peptide ib ntus muaj ob peb cysteine , lossis lwm cov amino acids reactive, uas yooj yim oxidized, Biorunstar muab xa cov peptide nrog cov cim ntawm lub zog reductant DTT. Cov peptide tsuas yog xa nrog DTT yog tias qhov no tau tso cai tshwj xeeb los ntawm cov neeg siv khoom.
Kuv yuav khaws kuv cov peptides hluavtaws li cas?
RE: Feem ntau lyophilized peptides yuav ruaj khov ntawm chav sov li 2-3 lub lis piam. Txhawm rau khaws cia ntev, koj yuav tsum khaws cov lyophilized peptides ntawm -20 degree . Kev rov ua kom khov-thaw yuav tsum zam. Cia kom tuaj rau hauv chav sov ua ntej qhib. Lub txee lub neej ntawm cov tshuaj peptide yog txwv; ib qho tshuaj peptide ib zaug npaj yuav tsum tau siv sai li sai tau.
Dab tsi yog qhov purity rau crude peptide thiab desalted peptide? Koj ua li cas ntxuav cov peptide? Dab tsi yog cov impurities?
RE: Rau cov peptides luv luv nrog cov kab ke ib txwm qis dua 15aa, feem ntau yog 40-60% los ntawm HPLC rau qib crude; 50-70% los ntawm HPLC rau qib desalted. Qhov ntev ntawm peptide, qhov qis dua qhov purity rau crude lossis desalted. Peptides feem ntau yog ntxuav los ntawm HPLC siv dej thiab acetonitrile gradient. Feem ntau cov impurities yog cov khoom tawg lossis tshem tawm peptides, tsis tiav de-tiv thaiv peptides, thiab cov ntsev thiab dej seem.
Kev piav qhia ntawm Peptide Purity los ntawm HPLC, Tag Nrho Cov Ntsiab Lus Peptide thiab Cov Ntsiab Lus Peptide.
RE: Biorunstar nkoj peptides raws li qhov hnyav tag nrho ntawm lyophilized hmoov. Cov hmoov lyophilized muaj xws li impurities xws li fragments lossis deletion peptides, tsis tiav de-tiv thaiv peptides, TFA thiab cov dej seem. Peptide Purity yog ntsuas los ntawm HPLC. Nws yog tus nqi ntawm peptide raug txheeb ze rau tag nrho cov analytes uas nqus ntawm ~ 214 nm (peptide daim ntawv cog lus absorbs), feem ntau yuav deletion, truncation los yog ua tsis tiav de-protected sequences, thiab lwm yam. Peptide purity los ntawm HPLC tsis suav nrog dej thiab ntsev. uas feem ntau muaj nyob rau hauv cov qauv. Tag nrho Peptide Cov ntsiab lus yog feem pua ntawm tag nrho cov peptides tam sim no txheeb ze rau txhua yam tam sim no hauv lyophilized peptide hmoov. Tag nrho cov ntsiab lus peptide yog txiav txim siab los ntawm kev tsom xam cov ntsiab lus nitrogen. Lub hom phiaj Peptide Cov ntsiab lus hauv lyophilized peptide hmoov yog li tuaj yeem xaus lus los ntawm cov mis: Tag Nrho Peptide Cov Ntsiab Lus X Peptide Purity los ntawm HPLC.
Txoj kev ntawm Fluorescein labelling yog dab tsi?
RE: FITC (Fluorescein isothiocyanate) yog lub tshuab ua ntej siv rau Fluorescein labelling. Rau qhov ua tau zoo Nterminal labelling, xya-atom aminohexanoyl
spacer (NH2-CH2-CH2-CH2-CH2-CH2-COOH) yog muab tso rau ntawm fluorophore (fluoroscein) thiab N-terminus ntawm peptide. Qhov no spacer pab cais cov fluorophore los ntawm nws cov ntsiab lus txuas, muaj peev xwm txo qis kev sib cuam tshuam ntawm fluorophore nrog cov biomolecule uas nws yog conjugated thiab ua rau nws nkag mus rau cov tshuaj ntsuam xyuas thib ob.
spacer (NH2-CH2-CH2-CH2-CH2-CH2-COOH) yog muab tso rau ntawm fluorophore (fluoroscein) thiab N-terminus ntawm peptide. Qhov no spacer pab cais cov fluorophore los ntawm nws cov ntsiab lus txuas, muaj peev xwm txo qis kev sib cuam tshuam ntawm fluorophore nrog cov biomolecule uas nws yog conjugated thiab ua rau nws nkag mus rau cov tshuaj ntsuam xyuas thib ob.
Puas yog C-terminal labeling ntawm Biotin (lossis FITC) ua tau?
RE: Yog. C-terminal labeling ntawm Biotin (lossis FITC) yog ua los ntawm kev sib ntxiv ntawm Lysine residue ntawm C-terminus ntawm peptide, thiab Biotin (lossis FITC) txuas rau Lysine sab saw ntawm amide daim ntawv cog lus. Lysine tus nqi zoo raug tshem tawm.
Dab tsi yog qhov tsim nyog peptide ntev rau kev tsim cov tshuaj tiv thaiv kab mob?
RE: Feem ntau, ib qho 10-25 residue peptide raug pom zoo. Lub peptide ntev dua tuaj yeem muaj cov epitopes ntau dua, tab sis kuj tseem tuaj yeem muaj lub sijhawm ntau dua ntawm kev tsim cov qauv hauv nruab nrab uas tsis yog cov ntaub ntawv ib txwm muaj. Ib qho luv luv peptide (<10aa) is generally not good unless there are valid reasons for it, such as potential sequence homology with a related family member or other proteins.
MAP yog dab tsi?
RE: MAPS los yog Multi-Antigenic Peptide yog ib tug ceg peptide uas linear peptide chains txuas ntawm lawv C-terminus ntawm polylysine core, yog li ua rau qhov loj ntawm tag nrho cov molecule. Qhov no yog ua kom tshem tawm cov coupling ntawm peptides rau KLH. Nws zoo nkaus li tias, txawm li cas los xij, kev sib haum xeeb ntawm peptides ntawm MAP yog qhov hloov pauv tsawg, thiab cov tshuaj tiv thaiv tau los ntawm MAP feem ntau lees paub cov hom phiaj protein tsawg dua li cov qauv KLH conjugation. Tsis tas li ntawd, tsis muaj peptide dawb tsim thaum ua MAPS, ua rau nws nyuaj rau tshem tawm polylysine core directed antibodies. Kev lim dej ntawm MAPS los ntawm HPLC yog qhov nyuaj, thiab MAPS yog muab tsis muaj kev pov thawj loj vim nws cov heterogeneity thiab loj molecular loj.
Vim li cas kuv cov tshuaj KLH-conjugated peptide tshwm sim huab?
RE: KLH los yog Keyhole Limpet Hemocyanin yog cov protein ntau (MW=4x105 - 1x107). Vim nws qhov loj thiab cov qauv, nws solubility hauv dej yog txwv, ua rau pom huab. Qhov no yuav tsis cuam tshuam rau immunogenicity thiab cov tshuaj turbid tuaj yeem siv rau kev txhaj tshuaj.
Koj puas tuaj yeem piav qhia M + Na thiab M + K huab hwm coj peaks hauv MALDI spectra?
RE: Nws yog heev pom Na (sodium) thiab K (potassium) adducts hauv MALDI spectrum. Cov sodium thiab potassium yog los ntawm cov dej siv hauv cov kuab tshuaj peptide. Txawm tias cov dej distilled thiab deionized muaj cov lej ntawm sodium thiab potassium ions, uas tsis tuaj yeem raug tshem tawm tag nrho. Cov no ua ionized thaum lub sij hawm MALDI huab hwm coj spec txheej txheem thiab khi rau cov dawb carboxyl pawg ntawm peptide. Vim tias tsis muaj cov dej huv huv uas yuav tshem tawm txhua qhov sodium lossis potassium ion los ntawm dej, pom cov sodium thiab potassium adducts nyob rau lub sijhawm muaj ntau heev thiab tsis tuaj yeem zam hauv MALDI huab hwm coj spec. Qhov no tsis yog qhov qhia tau tias peptide tsis huv, thiab nws yuav tsum tsis meej pem nrog qhov hnyav molecular tsis raug.