Hybridoma Cell Expansion And Subcloning Medium

产品信息 Product Description

产品名称

Khoom npe

杂交瘤细胞扩培及亚克隆专用培养基

Hybridoma Cell Expansion thiab Subcloning Medium

货号 Cat. No

BRS-MED02

规格

Specification

500ml / hli

500 mL / lub raj mis

产品组分

Cheebtsam

DMEM cov nyiaj tau los: 80%

DMEM Base nruab nrab: 80% (v/v)

进口胎牛血清:10%(体积比)

Fetal Bovine Serum (FBS, imported): 10% (v/v)

条件培养基:10%(体积比)

Qhov nruab nrab nruab nrab: 10% (v / v)

Qhov hnyav: 100 单位/ml

Penicillin: 100 U / ml

Qhov hnyav: 100 单位/ml

Streptomycin: 100 U / ml

其它添加物:单细胞增殖因子及小分子添加剂

Lwm yam Additives: Ib qho -cov xov tooj ntawm tes proliferation thiab cov tshuaj molecule me me

保存条件

Cia tej yam kev mob

-20℃保存, 有效期 18 个月.

Khaws ntawm -20 degree, txee lub neej 18 lub hlis

使用方法

Cov lus qhia rau kev siv

培养基融化:

37℃水浴融化本培养基, 乙醇消毒外表面后置超净台备用;

Daim ntawv thov: 本品融化后出现少量白色絮状物属正常现象, 3-5min 使其

沉至瓶底,然后吸取上清使用即可.

Thawing lub Medium:

Thawb qhov nruab nrab hauv ib da dej 37 degree. So sab nrauv nrog 70% ethanol thiab tso rau hauv lub txee biosafety rau kev siv.
Lus Cim: Cov tsos ntawm me me ntawm cov khoom siv dawb flocculent tom qab thawing yog qhov qub. Cia qhov nruab nrab sawv ntsug rau 3-5 feeb rau cov flocs kom khom, ces sau cov supernatant siv.

融合孔处理(状态良好细胞):

针对状态良好的融合孔(比如孔内培养基颜色正常或轻微发黄,且显微镜下细胞状态良好,透亮,大小正常等) 可直接使用黄枪头轻轻吹打融合孔, 细胞计数后按0.8-1.5cell/孔/200ul培养基.

Tuav Fusion Wells – Healthy Cells:

Rau fusion qhov dej nrog cov hlwb zoo (piv txwv li, xim nruab nrab lossis daj me ntsis, cov hlwb hauv lub tshuab ntsuas pom pom kev noj qab haus huv, ci ntsa iab, thiab qhov loj me), maj mam muab tso rau ntawm lub taub dej daj. Suav cov hlwb thiab phaj ntawm 0.8-1.5 hlwb / zoo hauv 200 µL nruab nrab.

融合孔处理(状态欠佳细胞):

针对状态不太良好的融合孔(比如孔内培养基颜色酸化发黄或显微镜下细胞状态皱缩,发黑等)可选择2种方式进行后续亚克隆.

Tuav Fusion Wells - Cov Cells Zoo Tshaj Plaws:

Rau fusion qhov dej nrog cov hlwb nyob rau hauv qhov zoo tshaj plaws (piv txwv li, nruab nrab acidified / daj lossis hlwb tshwm sim shriveled, tsaus nti hauv lub tshuab ntsuas), muaj ob txoj kev xaiv rau subcloning tom ntej:

第一种方式是将融合孔细胞吸至装有1ml本培养基的24孔板内,在扩培后的第2-3日再行计数进行亚克隆铺板(0. 8-1.5cell / 孔 / 200ul 培养基), 此时融合孔细胞倍增的次数有限且细胞状态良好, 非常适䓎

Kev xaiv 1: Hloov cov hlwb los ntawm fusion zoo rau hauv 24-zoo phaj uas muaj 1 mL ntawm qhov nruab nrab no. Tom qab 2-3 hnub ntawm kev nthuav dav, suav cov hlwb thiab ua subcloning ntawm 0.8-1.5 hlwb / zoo hauv 200 µL nruab nrab. Nyob rau theem no, cell doubling yog txwv tab sis cell mob yog zoo, ua rau nws zoo tagnrho rau subcloning.

第2种方式是使用黄枪头小心吸弃融合孔上清, 每孔补充 200ul新鲜的本培养基, 5% 3 CO2 静置培养3-4小时, 然后取样细胞计数后进行亚克隆(0.8-1.5cell/孔/200ul培养基);

Ua tib zoo tshem tawm cov supernatant los ntawm fusion zoo siv lub taub hau daj thiab ntxiv 200 µL ntawm qhov nruab nrab tshiab rau ib qhov dej. Muab tso rau ntawm 37 degree, 5% CO₂ rau 3-4 teev. Tom qab ntawd, ua piv txwv cov hlwb, suav, thiab ua subcloning ntawm 0.8-1.5 hlwb / zoo hauv 200 µL nruab nrab.

注:使用本司"杂交瘤扩培及亚克隆专用培养基"培养后的亚克隆一般第6-8日即可开始下游检测筛选.

Nco tseg: Subclones kab lis kev cai nrog Hybridoma Cell Expansion thiab Subcloning Medium feem ntau mus txog theem uas tsim nyog rau kev tshuaj ntsuam qis qis lossis kev tshuaj xyuas hnub 6–8 tom qab-subcloning.