Kho kom qhuav qhuav preservation
Kev ziab kom qhuav yog ib txoj hauv kev zoo rau khaws cia peptides. Ua peptides rau hauv cov hmoov qhuav qhuav thiab khaws cia rau ntawm -20 degree tuaj yeem tswj tau lawv qhov ruaj khov rau ntau xyoo, tsis txhob muaj cov kab mob degradation, cov qauv tsim, oxidation, lossis lwm yam kev hloov kho. Tsis tas li ntawd, khov-qhuav peptides tuaj yeem khaws cia rau ntau xyoo ntawm -10 degree lossis qis dua yam tsis muaj degradation.
Aseptic kuab tshuaj dissolution
Thaum dissolving peptides, nws raug nquahu kom siv cov dej tsis huv los yog cov dej distilled thiab yaj lawv nyob rau hauv cov kua qaub tsis muaj zog, uas tuaj yeem txo qhov degradation ntawm peptides los ntawm cov kab mob, kab mob, thiab proteases. Rau peptides uas muaj cov residues xws li Met, Cys, Trp, thiab lwm yam, vim lawv raug rau oxidation, nws raug nquahu kom yaj lawv hauv cov kuab tshuaj anaerobic thiab tau txais cov dej anaerobic los ntawm kev txhaj tshuaj inert gases (xws li nitrogen, helium, argon) hauv qab txo. siab.
Ntim thiab khaws cia
Txhawm rau txo qhov kev puas tsuaj los ntawm kev rov ua kom khov-thaw mus rau peptides, nws raug nquahu kom ua ntej pob peptide kuaj hauv cov fwj me me kom tsis txhob muaj ntau lub voj voog khov-thaw.
Tsis txhob muaj qhov chaw ntub dej
Peptides uas muaj Asp, Glu, Lys, Arg, los yog Nws yog nquag nqus dej los ntawm huab cua thiab ua deliques, yog li cov peptides yuav tsum tau muab cia rau hauv lub thawv kaw thiab qhuav.
Kev kho PH
Peptides muaj kev ruaj ntseg zoo nyob rau hauv acidic tej yam kev mob, thiab nws raug pom zoo kom pH ntau ntawm cov kuab tshuaj yog 3-6. Nws yog qhov zoo tshaj plaws rau pob thiab khov cov peptides yaj kom tsis txhob rov qab khov-thaw mus.
Lwm yam kev ceev faj
Rau peptides uas muaj Gln lossis Asn, lawv lub txee lub neej luv luv vim lawv yooj yim degradation.
Yog tias peptide yog hydrophobic heev vim muaj cov ntxhiab tsw qab xws li Val, Leu, Met, Phe, Tyr, Ala, lossis yog ib qho peptide nruab nrab, kev siv cov membrane denaturing agents xws li DMF lossis DMSO tuaj yeem pab yaj peptide. .
Hauv cov ntsiab lus, txhawm rau tswj kev ruaj ntseg ntawm peptides mus rau qhov loj tshaj plaws, nws raug nquahu kom siv cov txheej txheem khov-drying cia thiab sterile cov kuab tshuaj thaum lub sij hawm dissolution. Kev saib xyuas yuav tsum tau them rau kev kho tus nqi pH thiab ntim cia, thaum tsis txhob muaj qhov chaw ntub dej thiab rov ua kom khov-thaw mus.