Cov teeb meem thiab kev daws teeb meem hauv cov txheej txheem peptide synthesis suav nrog cov hauv qab no:
Cov ntsiab lus Peptide: Cov ntsiab lus Peptide yog hais txog feem pua ntawm cov tshuaj peptide hauv cov qauv piv rau cov tshuaj tsis peptide. Hauv cov khoom peptide, ntxiv rau peptide nws tus kheej, cov khoom tsis ntsev tau qhia thaum lub sijhawm tsim khoom, xws li dej, nqus cov kuab tshuaj, kev sib koom tes ions, thiab ntsev, kuj suav nrog.
Chemical stability:
Cov tshuaj tiv thaiv Deamidation: Asn / Gln residues tau yooj yim hydrolyzed los ua Asp / Glu.
Hydrolysis: Peptide bonds hauv peptides tau yooj yim tawg los ntawm hydrolysis, tshwj xeeb tshaj yog cov tsim los ntawm Asp kev koom tes, tshwj xeeb tshaj yog Asp Pro thiab Asp Gly peptide bonds.
Racemization cov tshuaj tiv thaiv: Tag nrho cov amino acid residues tshwj tsis yog Gly muaj chiral alpha carbon atoms thiab yog nquag rau racemization cov tshuaj tiv thaiv nyob rau hauv alkaline catalysis. Asp residues feem ntau ua rau cov tshuaj tiv thaiv racemization.
- tshem tawm degradation: Cys, Ser, Thr, Phe, Tyr thiab lwm yam residues tuaj yeem degraded los ntawm - tshem tawm, uas yog feem ntau tshwm sim ntawm alkaline pH thiab cuam tshuam los ntawm kub thiab hlau ions.
Lub cev stability:
Denaturation, adsorption, aggregation los yog nag lossis daus: Peptide denaturation feem ntau cuam tshuam nrog kev puas tsuaj ntawm cov txheej txheem tertiary thiab theem nrab. Nyob rau hauv lub xeev denatured, peptides muaj ntau dua rau cov tshuaj tiv thaiv thiab lawv cov kev ua ub no nyuaj rau rov zoo. Qhov nruab nrab tsim thaum lub sij hawm denaturation txheej txheem muaj tsawg solubility thiab yog nquag mus aggregation thiab nag lossis daus.
kev khaws cia:
Qhov zoo tshaj plaws cia tej yam kev mob: Peptides feem ntau khaws cia nyob rau hauv daim ntawv ntawm khov-qhuav hmoov, uas yog ruaj khov heev ntawm -20 degree, tshwj xeeb tshaj yog tom qab khov-qhuav thiab khaws cia nyob rau hauv ib tug -20 degree tshuab ziab khaub ncaws. Feem ntau peptides tuaj yeem khaws cia ntawm qhov ntsuas kub no tau ntau xyoo yam tsis muaj kev hloov pauv. Txhawm rau txo qhov cuam tshuam ntawm cov av noo, khov-qhuav peptides yuav tsum tau rov qab los rau hauv chav tsev kub hauv qhov chaw qhuav ua ntej yuav raug huab cua.
Ntim: Hesheng Biotechnology pom zoo kom ntim me me kom tsis txhob rov qab khov-thaw mus, txo tus naj npawb ntawm cov thawv qhib thiab kaw, yog li txo qhov kev pheej hmoo ntawm kev tuav tsis raug lossis cov kab mob sib kis, thiab ua kom muaj kev ruaj ntseg ntawm peptides.
Kev kuaj purity:
Purity guarantee: Tag nrho cov xa tawm peptides tau mus txog qhov xav tau purity, thiab peptides uas tsis ua raws li tus qauv yuav raug muab pov tseg. Cov txiaj ntsig HPLC thiab MS tuaj yeem txheeb xyuas qhov purity thiab molecular hnyav ntawm peptide, thaum pom cov impurities tseem ceeb.
Lwm txoj kev tshawb nrhiav, xws li kev txheeb xyuas cov amino acid lossis kev tshuaj ntsuam xyuas, tuaj yeem paub meej tias cov amino acid muaj pes tsawg leeg ntawm peptides yog ib qho kev qhia ntxiv ntawm peptide kev pom zoo.
Synthesis technology:
Khoom theem peptide synthesis: Khoom theem peptide synthesis tsis tas yuav ua kom huv ntawm cov khoom nruab nrab, tso cai rau cov txheej txheem txuas ntxiv thiab tso lub hauv paus rau kev ua haujlwm ntawm peptide synthesis. Raws li Fmoc tshuaj synthesis, lub hom phiaj peptide tau tsim los ntawm covalently txuas cov carboxyl pawg ntawm C-terminal amino acid nrog ib qho insoluble polymer resin, thiab tom qab ntawd pib los ntawm cov amino pawg ntawm cov amino acid.
Cov ntsiab lus saum toj no qhia txog cov teeb meem uas tshwm sim thiab kev tawm tsam hauv peptide synthesis, suav nrog ntau yam xws li tshuaj lom neeg ruaj khov, kev ruaj ntseg ntawm lub cev, kev cia khoom, kev kuaj purity, thiab lwm yam.