Peptide nucleic acid (PNA) yog ib qho khoom siv nucleic acid molecule uas muaj ntau yam zoo dua li cov DNA thiab RNA, xws li kev xaiv siab, muaj zog affinity, thiab stability. Cov theem khoom sib txuas yog ib txoj hauv kev tseem ceeb rau kev sib txuas PNA, nrog rau cov theem tshwj xeeb hauv qab no:
Xaiv cov pab pawg tiv thaiv tsim nyog: Ua rau cov pab pawg tiv thaiv nrog cov amino lossis carboxyl pawg ntawm PNA precursor los tsim cov esters lossis amides ntawm pawg tiv thaiv.
Txuas rau polymer resin: PNA precursor hloov nrog pab pawg tiv thaiv yog txuas nrog cov khoom siv polymer, feem ntau yog siv cov khoom siv polymer nrog cov roj carbon ntev ntawm 20-30 amino acid residues.
Rov ua dua lub voj voog synthesis: suav nrog tshem tawm N-terminal tiv thaiv pawg; Ntxiv rau chav tsev amino acid tom ntej; Ua N-terminal tiv thaiv dua.
Kev tiv thaiv thiab kev sib cais: Tom qab kev sib txuas, PNA peptide saw yog cais tawm ntawm cov khoom siv polymer los ntawm kev kho alkaline lossis acid hydrolysis, thiab tag nrho cov pab pawg tiv thaiv raug tshem tawm kom tau txais cov khoom PNA.
Txoj kev no tuaj yeem ua tau zoo sib xyaw PNA molecules txog li ob peb puas nucleotides ntev, thiab cov khoom siv hluavtaws muaj cov purity siab, ua rau lawv tsim nyog rau cov khoom loj.
Txawm li cas los xij, hauv kev siv tswv yim, qhov purity ntawm PNA sib sau ua ke los ntawm cov khoom siv hluav taws xob tsis siv neeg feem ntau tsis zoo tagnrho HiPep Research Institute tau tsim cov txheej txheem hluavtaws tshiab uas tuaj yeem muab cov khoom zoo PNA. Txoj kev no daws tau qee qhov teeb meem hauv kev sib xyaw ua ke, xws li cov khoom siv kim kim, yooj yim steric hindrance ntawm pab pawg tiv thaiv, thiab cuam tshuam tseem ceeb ntawm monomer purity ntawm cov khoom siv roj ntsha ntawm lub hom phiaj PNA Tus qauv synthesis ntau yog 10-15 hauv paus, thiab rau kev sib txuas ntawm ntau tshaj 16 lub hauv paus, nws yog ib qho kev txiav txim tshwj xeeb uas yuav tsum muaj kev sib cuag nrog tus neeg muag khoom
Tsis tas li ntawd, PNA synthesis kuj muaj ntau txoj kev hloov kho, xws li kev hloov kho fluorescence, biotin hloov, hloov pauv peptide, glycosylation, thiab DNA hloov kho, kom ua tau raws li cov kev xav tau ntawm kev tshawb fawb sib txawv, yog li ntawd, nws yog ib qho tseem ceeb heev uas yuav tsum xaiv cov txheej txheem synthesis tsim nyog thiab cov tswv yim raws li cov ntawv thov tshwj xeeb. scenarios thiab cov kev xav tau.
