Biotinylated Peptides

Ntawm Biorunstar, peb tshwj xeeb hauv kev tsim cov kev cai sib xyaw ntawm biotinylated peptides, muab siab - cov khoom siv huv huv raws li koj daim ntawv thov kev tshawb fawb tshwj xeeb. Peb cov kev pabcuam xyuas kom meej biotin labeling, pom kev purity, thiab txhim khu kev qha xa khoom, txhawb nqa ntau yam kev tshawb fawb.

 

Dab tsi yog Biotinylated Peptides?

Biotinylated peptides yog hluavtaws peptides hloov kho los ntawm kev txuas biotin (cov vitamin molecule me me, Vitamin B7) rau lawv cov qauv. Qhov biotin "tag" no ua rau muaj kev cuam tshuam tshwj xeeb nrog streptavidin lossis avidin proteins, uas khi biotin nrog kev sib raug zoo txawv txawv (ib qho ntawm cov tsis muaj zog tshaj tsis yog -covalent bonds hauv qhov). Qhov kev sib koom ua ke no tau siv dav hauv biotechnology thiab kev tshawb fawb biomedical rau kev kuaj pom, kev cais tawm, lossis kev tsis sib haum xeeb ntawm biomolecules.

 

Kev siv Biotinylated Peptides

Affinity Purification:Ncua thiab cais cov protein complexes

Kev Tshawb Fawb Kev Sib Tham:Ntsuam xyuas cov protein-peptide khi ntawm SPR, BLI, rub-kev soj ntsuam

Biosensors thiab Diagnostics:Txhim kho electrochemical thiab optical detection platforms (piv txwv li, SARS-CoV-2)

Cell Imaging thiab Targeting:Saib pom thiab taug qab peptide khi hauv cov hlwb

ELISA thiab Western Blot:Txhim kho rhiab heev thiab tshwj xeeb hauv immunoassays

 

Hauv kev tshawb fawb los ntawm Lin Liu et al. (2021), cov kws tshawb fawb tau tsim dual-kawg biotinylated peptide substrate nrog cov kab ke Biotin-GDEVDGK-Biotin, qhov twg GDEVD ua haujlwm raws li qhov chaw paub tshwj xeeb rau Caspase-3. Qhov kev ua haujlwm no qhia txog kev siv tshiab ntawm biotinylated peptides hauv protease-based biosensors.

Xia, N., Sun, Z., Ding, F., Wang, Y., Sun, W., & Liu, L. (2021). Protease biosensor los ntawm kev hloov dua siab tshiab ntawm homogeneous assay rau hauv qhov chaw -tethered electrochemical tsom xam raws li kev sib cuam tshuam ntawm streptavidin-biotin. ACS Sensors, 6(3), 1017–1024.https://doi.org/10.1021/acssensors.0c02408

 

Hauv kev tshawb fawb los ntawm Guohua Yi et al. (2021), pab pawg tshawb fawb tau ua haujlwm phage zaub thev naus laus zis los txheeb xyuas cov kab mob peptide uas tshwj xeeb khi rau cov receptor-binding domain (RBD) ntawm SARS-CoV-2. Txhawm rau ntsuas kev ua tau zoo ntawm kev sib khi ntawm cov peptides ntawm hauv vitro platforms, peptides tau hloov kho los ntawm biotinylation. Cov txiaj ntsig biotinylated peptides tom qab ntawd immobilized rau ntawm qhov chaw coated nrog streptavidin, xws li SPR sensor chips lossis cov hlaws sib nqus, yog li ua rau kev tsim kho ntawm qhov chaw ruaj khov thiab txhim khu kev qha.

Yaj, F.; Li, L.; Neuenschwander, PF; Idell, S.; Vankaalapati, R.; Jain, KG; Du, K.; Ji, H.; Yi, G. Phage Display-Derived Peptide for the Specific Binding of SARS-CoV-2. ACS Omega2021, 7 (4), 1210–1217. https://doi.org/10.1021/acsomega.1c05327

Peptide Biotinylation Services

Biorunstar muab cov kev xaiv biotinylation hloov tau ntawm ntau txoj haujlwm hauv peptide ib ntus kom tau raws li kev xav tau ntau yam kev sim. Peb muab biotinylation ntawm N-terminus, C-terminus, lossis sab hauv ntawm Lysine sab chains.

Feature	Paub meej
Customizable Biotin Qhov Chaw	- Biotin-N-terminus - Biotin-Ahx-N-terminus - Lys(Biotin)-Internal - Lys(Ahx-Biotin)-Internal - Lys(Biotin)-C-terminus - Lys(Ahx-Biotin)-C-terminus
Flexible Linkers	PEG, Ahx, lossis lwm yam kev cai txuas raws li xav tau
Purity	Ntau dua lossis sib npaug li 95% los ntawm HPLC
Analytical validation	HPLC chromatogram thiab Mass Spectrometry (MS) qhia
Lub sijhawm hloov pauv	Feem ntau 1-3 lub lis piam nyob ntawm qhov ntev ntawm peptide thiab qhov sib lawv liag complexity

Biotinylated Peptides Case Study

Biorunstar tau ua tiav ib qho ntawm biotinylated peptides nrog cov kab ke hauv qab no:

Ib ntus:Biotin-YGRKKRRQRRR-EXXXXXXYGXXXXXXPXXXR

Ntev:31 ua

M.W: 4145.89

HPLC Kev Ntsuam Xyuas:

MS Spectrum:

 

Cov peptide no tau ua tiav qhov purity ntawm 95.68% thiab tau xa tuaj tsis pub dhau 20 hnub ua haujlwm, qhia peb txoj kev mob siab rau kev ua tau zoo thiab kev ua haujlwm zoo.

Cov lus nug nquag

Q:Dab tsi yog lub sijhawm hloov pauv rau biotinylated peptides synthesis?
A:Peb lub sijhawm hloov pauv tus qauv yog 2-3 lub lis piam, nyob ntawm qhov nyuaj ntawm peptide.

Q:Kuv tuaj yeem xaiv qhov chaw ntawm biotinylation?
A:Yog lawm, peb muab N-terminal, C-terminal, thiab sab-chain biotinylation xaiv.

 

Xav tau ib nqe lus?Tiv tauj peb:sales@biorunstar.comTau txais cov lus hais hnub no!

Cim npe nrov: biotinylated peptides, biotinylated peptides lwm tus neeg