Tom qab nkag siab txog cov laj thawj rau peptide instability, paub tseeb raws li xyoo ntawm kev paub hauv peptide synthesis tias muaj cov hauv qab no cov kev daws teeb meem los txhim kho peptide stability.
1) Site directed mutation
Hloov cov residues uas ua rau peptide instability los yog qhia cov residues uas nce peptide stability los ntawm genetic engineering tuaj yeem txhim kho peptide stability.
2) Kev hloov kho tshuaj
Muaj ntau txoj kev hloov kho tshuaj rau peptides, nrog rau kev hloov kho PEG yog qhov feem ntau kawm. PEG yog cov dej-soluble polymer compound uas yog biodegradable thiab tsis muaj tshuaj lom hauv vivo. PEG tuaj yeem txhim kho cov solubility ntawm peptides tom qab khi, tswj biocompatibility, txhim khu thermal stability, tiv thaiv protease degradation, txo cov antigenicity, thiab ntev ib nrab-lub neej nyob rau hauv vivo. Xaiv txoj kev hloov kho kom tsim nyog thiab tswj cov qib ntawm kev hloov kho tuaj yeem txhim kho lub cev muaj zog lossis txhim kho kev ua haujlwm lom neeg qub.
3) Additives
Los ntawm kev ntxiv cov khoom ntxiv xws li qab zib, polyols, gelatin, amino acids, thiab qee cov ntsev, kev ruaj ntseg ntawm peptides tuaj yeem txhim kho. Qab Zib thiab polyols yuam kom cov dej molecules ntau nyob ib puag ncig cov proteins ntawm qhov tsis tshua muaj siab, yog li txhim kho kev ruaj ntseg ntawm peptides. Thaum lub sij hawm khov-drying txheej txheem, cov khoom uas tau hais los saum toj no kuj tuaj yeem hloov cov dej thiab tsim cov ntawv cog lus hydrogen nrog peptides kom ruaj khov rau lawv cov kev sib raug zoo, thiab tuaj yeem ua rau cov iav hloov pauv kub ntawm cov khoom khov-qhuav. Tsis tas li ntawd, surfactants xws li SDS, Tween, Pluronic tuaj yeem tiv thaiv peptide nto adsorption, aggregation, thiab nag lossis daus.
4) Khov kom qhuav
Cov tshuaj tiv thaiv kab mob uas tshwm sim hauv peptides, xws li deamidation, beta tshem tawm, hydrolysis, thiab lwm yam, xav tau kev koom tes ntawm dej, uas tuaj yeem ua haujlwm raws li theem mobile rau lwm cov reactants. Tsis tas li ntawd, qhov txo qis hauv cov ntsiab lus dej tuaj yeem ua rau qhov kub thiab txias ntawm peptides. Yog li ntawd, khov-qhuav tuaj yeem txhim kho kev ruaj ntseg ntawm peptides.